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Expedeon - now Abcam
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Ask Alex - Main considerations when using Lightning-Link Antibody conjugation kit
Alex - Expedeon's technical support specialist, speaks about the main considerations when using Lightning-Link Antibody conjugation kits. Find more about what the kit is used for, buffer considerations and the optimal antibody concentration for conjugation.
Переглядів: 370

Відео

InstantBlue - The fastest and the easiest to use Coomassie stain on the market
Переглядів 2594 роки тому
See why InstantBlue is the fastest and the easiest to use Coomassie stain and get a sample to try it out!
What is the biggest pain-point for our users and how does Lightning-Link® solve it?
Переглядів 664 роки тому
What is the biggest pain-point for our users and how does Lightning-Link® solve it?
What does Lightning-Link® mean to you, and what is the benefit it provides to the user?
Переглядів 3574 роки тому
What does Lightning-Link® mean to you, and what is the benefit it provides to the user?
Where do you see the future is going in your field?
Переглядів 874 роки тому
Where do you see the future is going in your field?
CaptSure™ DIY ELISA - Fastest ELISA development without need for plate coating
Переглядів 9905 років тому
CaptSure DIY ELISA is the latest cutting-edge ELISA kit with a built-in system for antibody labeling, enabling the rapid and easy development of in-house ELISAs using any antibody pair while eliminating the need to perform plate coating procedures. Find out more from www.expedeon.com/products/assays-antibodies/elisa-assays/captsure-diy-elisa/
Single incubation ELISA in 90 minutes!
Переглядів 3315 років тому
Discover how you can perform an ELISA quickly, easily and accurately with ELISA-ONE™ bit.ly/2vxqPSt
What's it like to work at Expedeon?
Переглядів 7535 років тому
Watch our latest video to discover what it's like to work for Expedeon directly from our staff!
See how you can stain your protein of interest in just 15 minutes with our InstantBlue™ solution!
Переглядів 1,6 тис.5 років тому
InstantBlue™ is a ready to use Coomassie protein stain for polyacrylamide gels. Its unique mechanism of action stains proteins in 15 minutes while leaving a clear background eliminating the need to fix, wash or destain. Formulated for safe use and easy disposal, its ready for use straight out of the bottle and comes in convenient pre-mixed 1 litre volumes. Perfect for use with the InstantBlue™ ...
Confirm the success of your antibody conjugation in just 10 minutes!
Переглядів 1,6 тис.5 років тому
See how quick & easy it is to confirm that your antibody has successfully conjugated to your label with our Check&Go! - If you are interested to learn more about this product range, please visit: bit.ly/2ORAw68 - If you are interested in learning how to conjugate a primary antibody directly, download our free guide: bit.ly/2zzARoK
TruePrime™ Webinar: a unique primer-free MDA technology for genomic DNA amplification
Переглядів 4466 років тому
See how you can overcome the problems and limitations of DNA amplification when using random primers. View our webinar and learn how you can eliminate primer artifacts, reduce amplification bias, and increase genome coverage. Learn more about our TruePrime™ technology by visiting bit.ly/2KjlFDa
How to label your primary antibody using Lightning-Link® technology
Переглядів 3 тис.6 років тому
Stop using secondary antibodies in immunoassays! Save time and materials by labeling your primary antibodies in under 20 min. For use in ELISA, Western blotting, immunochemistry, flow cytometry, and many other applications. Scientists love our Lightning-Link® kits because of the remarkable simplicity of the technology. Now they can open a Lightning-Link® Rapid kit, set up the reaction in 30 sec...
InnovaCoat® GOLD - gold nanoparticle conjugation technology
Переглядів 11 тис.6 років тому
Are you a scientist working with gold nanoparticles? InnovaCoat® GOLD products are 'conjugation friendly' nanoparticles with a proprietary surface coat that greatly enhances colloidal stability and permits easy covalent attachment of a variety of molecules, including antibodies, analytes, and other biomolecules. - If you are interested in our gold particles and gold nanoparticles conjugation ki...
Thunder-Link® conjugation kits for oligonucleotides and Immuno-PCR
Переглядів 1,8 тис.6 років тому
Thunder-Link® PLUS are easy to use oligonucleotide conjugation kits for antibodies and proteins for the use in R&D applications, drug discovery, and the development of diagnostic kits. Using standard chemistry and methodology, the linking of an oligonucleotide to molecules is difficult, time consuming and requires specialist knowledge. To overcome these issues the Thunder-Link® PLUS oligo conju...
Expedeon PiColorLock™ - non-radioactive phosphate detection kits
Переглядів 2156 років тому
Expedeon PiColorLock™ - non-radioactive phosphate detection kits
Expedeon Lightning-Link® antibody labeling technology
Переглядів 2,8 тис.6 років тому
Expedeon Lightning-Link® antibody labeling technology
Expedeon protein discovery & analysis - From sample preparation to protein visualization
Переглядів 1276 років тому
Expedeon protein discovery & analysis - From sample preparation to protein visualization
Gelfree™ 8100 Fractionation System - Let's Get Started
Переглядів 1,1 тис.6 років тому
Gelfree™ 8100 Fractionation System - Let's Get Started
TruePrime™ technology - Primer-free whole genome amplification
Переглядів 10 тис.6 років тому
TruePrime™ technology - Primer-free whole genome amplification
SYGNIS SunScript™ Webinar - A unique Reverse-Transcriptase to synthesize difficult RNAs
Переглядів 1396 років тому
SYGNIS SunScript™ Webinar - A unique Reverse-Transcriptase to synthesize difficult RNAs
Thunder-Link® PLUS Webinar: A guide to Immuno-PCR
Переглядів 10 тис.7 років тому
Thunder-Link® PLUS Webinar: A guide to Immuno-PCR
Lateral Flow Webinar: A Guide to Lateral Flow Immunoassay Development
Переглядів 50 тис.7 років тому
Lateral Flow Webinar: A Guide to Lateral Flow Immunoassay Development
Immunoassay Development Webinar: Fast, Simple and Cost-effective Immunoassay Development
Переглядів 2,3 тис.8 років тому
Immunoassay Development Webinar: Fast, Simple and Cost-effective Immunoassay Development
Press release: Innova Biosciences and Mologic Sign Supply and Licensing Agreement
Переглядів 1658 років тому
Press release: Innova Biosciences and Mologic Sign Supply and Licensing Agreement
Press release: New conjugation kits enable antibody labeling in less than 20 minutes
Переглядів 4099 років тому
Press release: New conjugation kits enable antibody labeling in less than 20 minutes
How to label your antibody using Lightning-Link® kits and stop using secondaries
Переглядів 11 тис.9 років тому
How to label your antibody using Lightning-Link® kits and stop using secondaries
Antibody-Nanoparticle Conjugation Webinar: Gold nanoparticles - optimization of conjugates
Переглядів 38 тис.9 років тому
Antibody-Nanoparticle Conjugation Webinar: Gold nanoparticles - optimization of conjugates
Antibody Purification Webinar: What you need to know to use antibodies effectively
Переглядів 28 тис.9 років тому
Antibody Purification Webinar: What you need to know to use antibodies effectively
Immunohistochemistry Webinar: An Introduction to Immunohistochemistry
Переглядів 75 тис.10 років тому
Immunohistochemistry Webinar: An Introduction to Immunohistochemistry
ELISA Webinar: An introduction to the basic principles and assay formats
Переглядів 48 тис.10 років тому
ELISA Webinar: An introduction to the basic principles and assay formats

КОМЕНТАРІ

  • @24hrsworship_chant
    @24hrsworship_chant Місяць тому

    🎯 Key points for quick navigation: 02:15 *🌈 Gold nanoparticles in the 10 to 200 nanometer range display a red color due to plasmon resonance.* 06:17 *🕵️‍♂️ Appearance differences of 40 nanometer gold from different suppliers are due to variations in shapes and sizes of the particles.* 08:44 *🏺 Absorbance profiles of gold nanoparticles can provide information on particle size, but mixed sizes can produce unexpected results.* 10:41 *🧬 Covalent linking of molecules to gold offers stability and allows for oriented coupling, unlike passive methods which result in random orientation.* 20:41 *🏷️ Covalent attachment to gold is more stable and allows for oriented coupling, enhancing performance in various applications.* 26:41 *💡 Optical sensing with gold nanoparticles can detect interactions between binding entities, leading to color changes for biosensing applications.* 28:40 *🎯 Gold nanoparticles can be used in drug delivery systems to reduce toxicity of drugs like cisplatin, and in photothermal therapy to target and kill tumors effectively.* Made with HARPA AI

  • @peacechinelo5180
    @peacechinelo5180 2 місяці тому

    Thank you for sharing this knowledge

  • @nikolai5012
    @nikolai5012 2 місяці тому

    How does the innovacoat bind to any antibody? So I could just add in NbALFA for example and it would naturally conjugate?

  • @devinmartin7626
    @devinmartin7626 2 місяці тому

    How would you field test for the presences of silver and gold nano particles in water ?

  • @haifaalmamari3670
    @haifaalmamari3670 3 місяці тому

    How we can prepare such coated gold nanoparticles for simulation work

  • @yuliatiherbani4990
    @yuliatiherbani4990 3 місяці тому

    the link isnt working

  • @user-cx7gc1eq4c
    @user-cx7gc1eq4c 5 місяців тому

    I love this, so informative and compact

  • @ukraniangandalf8885
    @ukraniangandalf8885 6 місяців тому

    Oxford university press has been awfuly quiet since this dropped 🔥 🔥 🔥

  • @ukraniangandalf8885
    @ukraniangandalf8885 6 місяців тому

    We making it out of the lab with this one 🔥 🗣️ 🗣️ 🔥

  • @sasia729
    @sasia729 11 місяців тому

    It might be quite late.....did u try with 5% skimmed milk in PBS as blocking buffer

  • @jimparsons6803
    @jimparsons6803 Рік тому

    Pretty slick. Heard that these objects can be used in organic catalytic chemistry too.

  • @alexlucassen8489
    @alexlucassen8489 Рік тому

    1.3 gw is about one conventional powerinstallatie as coal, nuclear., etc. So pretty much for only 1km of screen foil!!

  • @alexlucassen8489
    @alexlucassen8489 Рік тому

    Very interesting application, as it seems, for nano gold in solar panel applications in space. Their it is ALL about ‘KW to weight ratio performance’. Rocket lance costs mainly depend on the pay load. More kw pro kg load means less rocketlonges are needed to build the needed solar farms in space. This with the help of ‘rol in & rol of Solar Films’ to construct integrated zonscreens of around 1km2. Energy radiated by the son is around 1300w/m2. So the pontential is….~1,3 Gw pro km2 solar screen based on nanogold. Nanogold as explained increases the solar efficiency by a lot by this special and the ‘unique aliment absorption radiation effect’. All frequency are now absorbed better, besides a small band. Fantastic is our nature.

  • @SPHINXORMUS
    @SPHINXORMUS Рік тому

    I have an Ormus monatomic gold company in NZ " SPHINX ORMUS "we mix colloidal gold ruby red with our Ormus to increase the gold content and have it in both forms, colloidal gold has anti-inflammation properties. Ancient Egyptians took monatomic golf for longevity and higher spiritual consciousness

  • @firozsheikh3352
    @firozsheikh3352 Рік тому

    Very dull presentation..delete it pls

  • @prof1631
    @prof1631 Рік тому

    Please can you help me for where I get this machine and IGG,

  • @siskiyouecoforestfarmandsa1270
    @siskiyouecoforestfarmandsa1270 2 роки тому

    how is the nano gold covered 3rd dna strand being built inside of us? and that one nanometer thick gold sheathing for ioncreased conductivity....is this consensual?

  • @BenjaminJTemple4019
    @BenjaminJTemple4019 2 роки тому

    Hi @Kristina, what is your goal for watching this video?

  • @dmajumdar2059
    @dmajumdar2059 2 роки тому

    What a fantastic all-encompassing explanation! Thank you!

  • @BenjaminJTemple4019
    @BenjaminJTemple4019 2 роки тому

    @Kristina Roepke, How to thiolate an antibody? Do you know the answer to this and does this video relate to your studies on nursing or your bachelor's degree in microbiology or your minor in chemistry?

  • @abbasi1907
    @abbasi1907 2 роки тому

    The antigens despenses in the Strip can transmit infection if someone get contact with strip?

  • @marke.8334
    @marke.8334 2 роки тому

    If you all haven't seen a documentary called... It's worse than you think.. by revelations of Jesus Christ ministries.. I suggest you do... All praise and glory to the most high Jesus Christ

  • @pasupuletisrinivasarao6150
    @pasupuletisrinivasarao6150 2 роки тому

    Video not appear clearly

  • @BRYDN_NATHAN
    @BRYDN_NATHAN 2 роки тому

    Thank you. 8:04 the size attribute is 0.618 of the wave form 👍

  • @marke.8334
    @marke.8334 2 роки тому

    1- If you all haven't seen a documentary called... It's worse than you think.... By revelations of Jesus Christ ministries.... I suggest you do... All praise and glory to the most high Jesus Christ

  • @acm01864
    @acm01864 2 роки тому

    About time.....!

  • @prkunozowecmjkyjrtna7765
    @prkunozowecmjkyjrtna7765 2 роки тому

    text

  • @ywk7282
    @ywk7282 2 роки тому

    Thanks for the video I have a question regarding the gold nanoparticle. 1.What is the difference between Quantum dots and gold nano particles? 2. What is the emitting mechanism difference between Qds and plasmonic gold nanoparticle emission

  • @jurgmesser9779
    @jurgmesser9779 2 роки тому

    Is it possible to label 3 antibodies from the same host species (e.g. rabbit) separately with different Lightning-Link kits (e.g. Atto488, Cy5, AF647) and then mix the 3 labeled antibodies for an incubation all together at the same time?

  • @DoctorJellicle
    @DoctorJellicle 2 роки тому

    This video quality is rough

  • @armaceerulogos
    @armaceerulogos 2 роки тому

    Brilliant video! The lecture is really explainatory, and the topic is covered in a comprehensive way.

  • @aaabeverages7152
    @aaabeverages7152 3 роки тому

    Extremely interesting. A scientist used radiation nonionizing to heat preciousmetal particle invitro at a hospital several decades ago.

  • @williamlaureano3258
    @williamlaureano3258 3 роки тому

    CERN want to use it to live for ever , demonic

  • @dannypresley5353
    @dannypresley5353 3 роки тому

    Their going through all this math and technical formulas on the procedure of making the elixir of the God's when all they have to do is instantly vaporize the gold and it's the residue from the gold when it's vaporized it's a white powdery substance left over from vaporizing the gold you don't make a white powdery substance by using chemicals to break it down to nanoparticles lol by doing that their just taking the most important attributes away no no no double dog no that isn't the right way to make the right elixir of the God's

  • @dannypresley5353
    @dannypresley5353 3 роки тому

    Lol you know what's really gonna happen in time our governments of this world is gonna finally be able to travel from world to world and their gonna realize that earth is a very small world and not nearly the amount of people on it according to it's size and when they start construction on other worlds their not gonna have enough humans to do all this work just like what happened with the ancients and as a result they had to create us and that also will be another method to creating a strong work force and their also gonna have to figure out how to make folks live longer so they can produce more humans to grow their work force if earth didn't have so much water on it then it would have more sustainable land to grow more people you see the object isn't to survive it's to expand throughout the universe to go as far out into the universe as possible even beyond our known universe cause that's what our kind does we expand even the ancients did that you know how I know this ? It's exactly like this we are them just a smaller version

  • @dannypresley5353
    @dannypresley5353 3 роки тому

    Check this out ya'll do realize that the ancients also refined the gold to a fine fine practical and then sprinkled it in their atmosphere on their planet to hold in the warmth from their sun cause it depleting so these folks consume solid gold on a daily basis so as everyone can see these folks that's making this stuff now are only in it for the money their not actually selling us the elixir of the God's but it's good stuff not really trying to knock it cause it does have wonderful attributes it's just not actually the elixir of the God's

  • @dannypresley5353
    @dannypresley5353 3 роки тому

    It isn't made with nanoparticles it's actually made with the purest of gold and it's to be instantly vaporized and the remaining white powder is the substance left behind by the procedure lol you can't use ingredients that are almost like something to get the desired effects like that of the God's you must use the same stuff that they did yeah sure this stuff that their making now is definitely worth it and it's good for you but it isn't the elixir of the God's it's we want that then it's gonna cost a good bit of money but rest assured there's gonna come a time when medical is gonna be a real problem for our country so their gonna have to come up with cheaper ways to keep folks healthy and their gonna come to realize although the real elixir cost much more than these folks are making now but in the long run it would be cheaper for governments to just give folks the real elixir so they won't have to spend so much on health care mark my words it's all gonna come out in the wash

    • @TEXAS-SMITH
      @TEXAS-SMITH Рік тому

      Governments don't give a darn about you. Please understand that.

    • @SPHINXORMUS
      @SPHINXORMUS Рік тому

      Yea my company SPHINX ORMUS sells ormus mixed with colloidal gold , we energise and structure it using 8 types of subtle energy, sacredgeometry ,crytals,copper triskelions ,organite pyranids,copper numbian pyramids with crytals on apex , cobalt blue glass enhancing with the violet colour spectrum and paua shell which also emitts a electrucal charge .I might add i have tested and confirmed the electrical charge on an amp meter. When then harmonically imprint frequency into the liquid to bring it into a even higher vibrational tetrahedron state. Water being a sponge with memory passes benefits of the frequency when the gold is consumed. 8 hz 88hz 888hz Abundance, 40hz long term brain memory,100hz increased cognitive function,285hz influences energy field and cell repair,432hz manifestation,528hz miracle tone DNA repair,639hz attract love and positive energy,741 hz cellular detox,741 hz awaken intuition,963hz connection to god or universal energy. I can say this Ancient Egyptian organic elixir has healed many client and is proven to Boost the merkabah or Aura up to 120% running all 7 charkras at unblocked optimum energy for a maximum electrical being. We have Aura imaging evidence. Its pretty charged gold

  • @rogerinkeywest
    @rogerinkeywest 3 роки тому

    Thanks for your message.

  • @Sh4dowHunter42
    @Sh4dowHunter42 3 роки тому

    Okay, I get the benefits of using the gold, now I need to know why I would want to attach nano gold to proteins in the first place, wouldn't I just eat my chicken the same way I always have?

  • @juliemosley3814
    @juliemosley3814 3 роки тому

    So if you attach a COVID antibody to the coated gold you would have the cure? ??

  • @ptiyouthiya
    @ptiyouthiya 3 роки тому

    You have beautiful Voice. ! A girl with knowledge. ma sha Allah.

  • @user-eg8cb7er9e
    @user-eg8cb7er9e 3 роки тому

    Many thanks for well-structured information! Unfortunately, links to the guide and your products are unavalible. Could you please make te correction?

  • @Monster-cj7kf
    @Monster-cj7kf 3 роки тому

    How much colloidal gold?

  • @gerenciacontruyeperusac4282
    @gerenciacontruyeperusac4282 3 роки тому

    stupid "test" for stupid people. cormandrostenreview.com/report/ one beetween 1000 or 2000 studies, or more, from every part of the world. Covidiots.

  • @saleemhasmi7863
    @saleemhasmi7863 3 роки тому

    Hindi

  • @jens8408
    @jens8408 3 роки тому

    Ñtñthnyn

  • @chantalvachon1451
    @chantalvachon1451 3 роки тому

    Why is the covid test made of nanotechnology compagnies?? Like :Sona nanotech inc. gold nanoparticules Do we get gold nanoparticules injected in our nose? Why?

    • @EsotericGold_net
      @EsotericGold_net 3 роки тому

      The more nanoparticles placed in the body, the more toxins surround cells causing the body not to recognize it's own cells, causing autoimmune disease.

    • @nagpapataba
      @nagpapataba 2 роки тому

      The swabbing action doesn't add anything to your nose. It's just a cotton tip getting a sample of the fluid in your nose. Then whatever it gets is the thing being tested. Nanoparticles will be involved in the detection of the virus collected on the cotton tip. The nanoparticles allow testing to be efficient and accurate and we should be grateful to the nanotechnology companies for using their work to help control the pandemic

  • @rovstam7989
    @rovstam7989 3 роки тому

    The sound its terrible i can barely understand what she says

  • @zahramardy4056
    @zahramardy4056 3 роки тому

    Very informative, thank you

  • @rtod4
    @rtod4 3 роки тому

    I use the colloidal metals for health. They are effective against LPS producing bacteria. But there is precious little info of this type. Very fascinating talk - thank you!

    • @lisagrace6471
      @lisagrace6471 3 роки тому

      what is lps producing bacteria? Where did you learn about this? How do you use it this way?

    • @rtod4
      @rtod4 3 роки тому

      @@lisagrace6471 I wouldn't recommend the coloidal silver unless you can find someone to coach you, it can produce strong reactions, and if you're not ready for them, it can mess you up. Basically, for many of the modern diseases there is no cure (secret or otherwise). However, they are easily preventable if you start young. Low carb diets, probiotics, exercise, better dental care. Mostly the modern diseases are some form of bacterial biofilms, most likely from leaky gut or poor dental care, that migrate and take up residence in various places. If the bacteria take up in the pancreas, you get pancreatic diseases. If they end up in the joints, you end up with arthritis/gout or the dread 'autoimmune' disease. Now, to your question, LPS is Lippopolysaccharide, a signaling protein used by disease causing gram negative, and some gram positive bacteria. LPS, aka Endotoxin, is one of the most neurotoxin around. It's bad enough that your immune system (innate immune system) is already primed to react to the LPS protein. LPS is not used when these bacteria are free-living in the blood (planktonic), but when they are part of a biofilm community, the LPS protein is used for quorum signaling. The LPS (and other) toxic proteins then enter the bloodstream and triggers general inflammation in the blood stream. But the bacteria are completely protected from the immune system when they are in a biofilm. Most western doctors will say its bogus, but I challenge any of them to research it in PubMed, the NIH clearing house for peer reviewed research Go here and you'll find a bunch of peer reviewed papers telling what happens when oral bacterial finds its way to the pancreas. pubmed.ncbi.nlm.nih.gov/?term=oral+bacteria+pancreas&size=200 BTW - oral bacteria are just as nasty as Lyme. My own opinion for the people who are convinced they have Lyme but test negative is that they likely have gut or dental bacteria that have leaked into the rest of the body. They can be just as debilitating as the dreaded Lyme or Syphilis. In fact, Treponema denticola is a sibling of syphilis, and causes the same neurological symptoms as Lyme or Syphilis. Oh, fwiw, for every doctor who says colloidal silver doesn't work, I can find you two peer reviewed papers that says it does. Unfortunately, modern medicine doesn't listen to science. HTH

    • @biggieshorty
      @biggieshorty 3 роки тому

      @@rtod4 The reason I came to this video. I want to know how these things work or can become adverse for some. Thanks for your comments. Any more info you can provide. Channels you watch on this would be great.